dlp00 15 6 to 1000 Search Results


dlp00 15 6 to 1000  (R&D Systems)
94
R&D Systems dlp00 15 6 to 1000
Dlp00 15 6 To 1000, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
dlp00 15 6 to 1000 - by Bioz Stars, 2026-03
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leptin  (R&D Systems)
95
R&D Systems leptin
Figure 2. Differentiation of human primary adipocytes alters adiponectin and <t>leptin</t> release. Human primary adipocytes, differentiated at 21%, 10% or 5% O 2 , were incubated for 24 h in αMEM at the same oxygen conditions. Afterwards, supernatants were collected for <t>further</t> <t>ELISA</t> measurements and cells were harvested and directly lysed. Total lysates (10 µg) were resolved by 10% SDS-Page and analyzed via western blotting on polyvenylidenefluorid (PVDF) membranes and further processed as described in Materials and Methods. (A) Protein expression of adiponectin was detected by ECL. Representative blots are shown. Supernatants were analyzed for protein concentration of (B) adiponectin and (C) leptin. Data sets are mean values ± SEM of ≥ 4 independent experiments. *p ≤ 0.05 compared with the control at 21% O 2 .
Leptin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/leptin/product/R&D Systems
Average 95 stars, based on 1 article reviews
leptin - by Bioz Stars, 2026-03
95/100 stars
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Figure 2. Differentiation of human primary adipocytes alters adiponectin and leptin release. Human primary adipocytes, differentiated at 21%, 10% or 5% O 2 , were incubated for 24 h in αMEM at the same oxygen conditions. Afterwards, supernatants were collected for further ELISA measurements and cells were harvested and directly lysed. Total lysates (10 µg) were resolved by 10% SDS-Page and analyzed via western blotting on polyvenylidenefluorid (PVDF) membranes and further processed as described in Materials and Methods. (A) Protein expression of adiponectin was detected by ECL. Representative blots are shown. Supernatants were analyzed for protein concentration of (B) adiponectin and (C) leptin. Data sets are mean values ± SEM of ≥ 4 independent experiments. *p ≤ 0.05 compared with the control at 21% O 2 .

Journal: Adipocyte

Article Title: Differentiation of human adipocytes at physiological oxygen levels results in increased adiponectin secretion and isoproterenol-stimulated lipolysis

doi: 10.4161/adip.19962

Figure Lengend Snippet: Figure 2. Differentiation of human primary adipocytes alters adiponectin and leptin release. Human primary adipocytes, differentiated at 21%, 10% or 5% O 2 , were incubated for 24 h in αMEM at the same oxygen conditions. Afterwards, supernatants were collected for further ELISA measurements and cells were harvested and directly lysed. Total lysates (10 µg) were resolved by 10% SDS-Page and analyzed via western blotting on polyvenylidenefluorid (PVDF) membranes and further processed as described in Materials and Methods. (A) Protein expression of adiponectin was detected by ECL. Representative blots are shown. Supernatants were analyzed for protein concentration of (B) adiponectin and (C) leptin. Data sets are mean values ± SEM of ≥ 4 independent experiments. *p ≤ 0.05 compared with the control at 21% O 2 .

Article Snippet: Concentration of adiponectin (RD191023100; detection range 1–150 ng/ml) and VEGF-A (RBMS277/2R; detection range 15.6–1000 pg/ml) was determined with ELISA kits from Biovendor, IL-6 (950.030.192; detection range 6.25–200 pg/ml) and MCP-1 (650.110.192; detection range 16–1000 pg/ml) was determined using ELISA kits purchased from Gen-Probe and leptin (DLP00; detection range 15.6–1000 pg/ml) and DDP-4 (DC260; detection range 0.31–20 ng/ml) concentrations were analyzed by ELISA kits purchased from R&D Systems.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, SDS Page, Western Blot, Expressing, Protein Concentration, Control